Primer3 0.4.0 [portable]

Where i runs over Tm, GC%, length, product size, and complementarity scores. This allows the algorithm to return the best pair even if no pair satisfies all absolute constraints – a practical lifesaver for AT-rich or GC-rich templates.

. They paste in a raw DNA sequence—a string of A, C, G, and Ts—and hit "Pick Primers." The Output: primer3 0.4.0